Expressing:Article Title: ELF5 modulates casein synthesis in goat mammary epithelial cells via JAK2/STAT5 signaling pathway.
Article Snippet: Then the membrane incubated with the secondary antibody.Then the membrane incubated with the secondary antibody.. Primary antibodies contains ELF5 (sc-166653, SantaCruz; 1:1,000), αS2-casein (bs-10034R, Bioss; 1:2,000), κ-casein (bs-10031R; Bioss; 1:1,000), αS1-casein (bs-10033R; Bioss; 1:1,000), β-casein (sc-166530; SantaCruz; 1:1,000), pJAK2 (ab32101; Abcam; 1:1,000), p-STAT5 (9351S; 1:1,000), JAK2 (3230; CST; 1:1,000), STAT5 (610191; BD; 1:1,000), proliferating cell nuclear antigen (PCNA, 10205-2-APl Proteintech, 1:5,000), cyclin dependent kinase 2 (CDK2, 10122-1-AP; Proteintech, 1:10,000), B-cell lymphoma-2-associated X (BAX, 50599-2-lg; Proteintech, 1:10,000), Caspase3 (19677-1- AP; Proteintech, 1:2,000), β-Tubulin (CW0098M; CWBIO, 1:5,000).. Secondary antibody contains horseradish peroxidase-conjugated goat anti-rabbit-IgG (CW0102; CWBIO; 1:5,000) and goat anti-mouse-IgG (CW0103; CWBIO; 1:5,000).Secondary antibody contains horseradish peroxidase-conjugated goat anti-rabbit-IgG (CW0102; CWBIO; 1:5,000) and goat anti-mouse-IgG (CW0103; CWBIO; 1:5,000).
Article Title: ELF5 modulates casein synthesis in goat mammary epithelial cells via JAK2/STAT5 signaling pathway
Article Snippet: Then the membrane incubated with the secondary antibody.Then the membrane incubated with the secondary antibody.. Primary antibodies contains ELF5 (sc-166653, SantaCruz; 1:1,000), αS2-casein (bs-10034R, Bioss; 1:2,000), κ-casein (bs-10031R; Bioss; 1:1,000), αS1-casein (bs-10033R; Bioss; 1:1,000), β-casein (sc-166530; SantaCruz; 1:1,000), p-JAK2 (ab32101; Abcam; 1:1,000), p-STAT5 (9351S; 1:1,000), JAK2 (3230; CST; 1:1,000), STAT5 (610191; BD; 1:1,000), proliferating cell nuclear antigen (PCNA, 10205-2-APl Proteintech, 1:5,000), cyclin dependent kinase 2 (CDK2, 10122-1-AP; Proteintech, 1:10,000), B-cell lymphoma-2-associated X (BAX, 50599-2-lg; Proteintech, 1:10,000), Caspase3 (19677-1-AP; Proteintech, 1:2,000), β-Tubulin (CW0098M; CWBIO, 1:5,000).. Secondary antibody contains horseradish peroxidase-conjugated goat anti-rabbit-IgG (CW0102; CWBIO; 1:5,000) and goat anti-mouse-IgG (CW0103; CWBIO; 1:5,000).Secondary antibody contains horseradish peroxidase-conjugated goat anti-rabbit-IgG (CW0102; CWBIO; 1:5,000) and goat anti-mouse-IgG (CW0103; CWBIO; 1:5,000).
Article Title: Negative regulation of α S1 -casein (CSN1S1) improves β-casein content and reduces allergy potential in goat milk.
Article Snippet: membranes were washed with TBST 3 times, incubated with secondary antibodies. .. Secondary antibody horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (CW0103, CW Biotech; Beijing, China; 1:5,000) was used for primary antibodies αS1-casein (SAB1401093, Sigma-Aldrich; 1:1,000), αS2-casein (bs-10034R, Bioss, Beijing, China; 1:500), β-casein (orb2053, Biorbyt, Cambridgeshire, UK; 1:500), κ-casein (GTX60145, GeneTex, Irvine, CA; 1:500), JAK2 (3230, Cell Signaling Technology, Danvers, MA; 1:1,000), p-JAK2 Tyr1007/1008 (ab32101, Abcam, Cambridge, UK; 1:1,000), mTOR(2972, Cell Signaling Technology; 1:1,000) and p-mTOR Ser2448 (2971; Cell Signaling Technology; 1:1,000). .. Secondary antibody HRP-conjugated goat anti-mouse IgG (CW0102, CW Biotech; 1:5,000)
Transfection:Article Title: ELF5 modulates casein synthesis in goat mammary epithelial cells via JAK2/STAT5 signaling pathway.
Article Snippet: Then the membrane incubated with the secondary antibody.Then the membrane incubated with the secondary antibody.. Primary antibodies contains ELF5 (sc-166653, SantaCruz; 1:1,000), αS2-casein (bs-10034R, Bioss; 1:2,000), κ-casein (bs-10031R; Bioss; 1:1,000), αS1-casein (bs-10033R; Bioss; 1:1,000), β-casein (sc-166530; SantaCruz; 1:1,000), pJAK2 (ab32101; Abcam; 1:1,000), p-STAT5 (9351S; 1:1,000), JAK2 (3230; CST; 1:1,000), STAT5 (610191; BD; 1:1,000), proliferating cell nuclear antigen (PCNA, 10205-2-APl Proteintech, 1:5,000), cyclin dependent kinase 2 (CDK2, 10122-1-AP; Proteintech, 1:10,000), B-cell lymphoma-2-associated X (BAX, 50599-2-lg; Proteintech, 1:10,000), Caspase3 (19677-1- AP; Proteintech, 1:2,000), β-Tubulin (CW0098M; CWBIO, 1:5,000).. Secondary antibody contains horseradish peroxidase-conjugated goat anti-rabbit-IgG (CW0102; CWBIO; 1:5,000) and goat anti-mouse-IgG (CW0103; CWBIO; 1:5,000).Secondary antibody contains horseradish peroxidase-conjugated goat anti-rabbit-IgG (CW0102; CWBIO; 1:5,000) and goat anti-mouse-IgG (CW0103; CWBIO; 1:5,000).
Article Title: ELF5 modulates casein synthesis in goat mammary epithelial cells via JAK2/STAT5 signaling pathway
Article Snippet: Then the membrane incubated with the secondary antibody.Then the membrane incubated with the secondary antibody.. Primary antibodies contains ELF5 (sc-166653, SantaCruz; 1:1,000), αS2-casein (bs-10034R, Bioss; 1:2,000), κ-casein (bs-10031R; Bioss; 1:1,000), αS1-casein (bs-10033R; Bioss; 1:1,000), β-casein (sc-166530; SantaCruz; 1:1,000), p-JAK2 (ab32101; Abcam; 1:1,000), p-STAT5 (9351S; 1:1,000), JAK2 (3230; CST; 1:1,000), STAT5 (610191; BD; 1:1,000), proliferating cell nuclear antigen (PCNA, 10205-2-APl Proteintech, 1:5,000), cyclin dependent kinase 2 (CDK2, 10122-1-AP; Proteintech, 1:10,000), B-cell lymphoma-2-associated X (BAX, 50599-2-lg; Proteintech, 1:10,000), Caspase3 (19677-1-AP; Proteintech, 1:2,000), β-Tubulin (CW0098M; CWBIO, 1:5,000).. Secondary antibody contains horseradish peroxidase-conjugated goat anti-rabbit-IgG (CW0102; CWBIO; 1:5,000) and goat anti-mouse-IgG (CW0103; CWBIO; 1:5,000).Secondary antibody contains horseradish peroxidase-conjugated goat anti-rabbit-IgG (CW0102; CWBIO; 1:5,000) and goat anti-mouse-IgG (CW0103; CWBIO; 1:5,000).
Article Title: Negative regulation of α S1 -casein (CSN1S1) improves β-casein content and reduces allergy potential in goat milk.
Article Snippet: membranes were washed with TBST 3 times, incubated with secondary antibodies. .. Secondary antibody horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (CW0103, CW Biotech; Beijing, China; 1:5,000) was used for primary antibodies αS1-casein (SAB1401093, Sigma-Aldrich; 1:1,000), αS2-casein (bs-10034R, Bioss, Beijing, China; 1:500), β-casein (orb2053, Biorbyt, Cambridgeshire, UK; 1:500), κ-casein (GTX60145, GeneTex, Irvine, CA; 1:500), JAK2 (3230, Cell Signaling Technology, Danvers, MA; 1:1,000), p-JAK2 Tyr1007/1008 (ab32101, Abcam, Cambridge, UK; 1:1,000), mTOR(2972, Cell Signaling Technology; 1:1,000) and p-mTOR Ser2448 (2971; Cell Signaling Technology; 1:1,000). .. Secondary antibody HRP-conjugated goat anti-mouse IgG (CW0102, CW Biotech; 1:5,000)
Quantitative Proteomics:Article Title: ELF5 modulates casein synthesis in goat mammary epithelial cells via JAK2/STAT5 signaling pathway.
Article Snippet: Then the membrane incubated with the secondary antibody.Then the membrane incubated with the secondary antibody.. Primary antibodies contains ELF5 (sc-166653, SantaCruz; 1:1,000), αS2-casein (bs-10034R, Bioss; 1:2,000), κ-casein (bs-10031R; Bioss; 1:1,000), αS1-casein (bs-10033R; Bioss; 1:1,000), β-casein (sc-166530; SantaCruz; 1:1,000), pJAK2 (ab32101; Abcam; 1:1,000), p-STAT5 (9351S; 1:1,000), JAK2 (3230; CST; 1:1,000), STAT5 (610191; BD; 1:1,000), proliferating cell nuclear antigen (PCNA, 10205-2-APl Proteintech, 1:5,000), cyclin dependent kinase 2 (CDK2, 10122-1-AP; Proteintech, 1:10,000), B-cell lymphoma-2-associated X (BAX, 50599-2-lg; Proteintech, 1:10,000), Caspase3 (19677-1- AP; Proteintech, 1:2,000), β-Tubulin (CW0098M; CWBIO, 1:5,000).. Secondary antibody contains horseradish peroxidase-conjugated goat anti-rabbit-IgG (CW0102; CWBIO; 1:5,000) and goat anti-mouse-IgG (CW0103; CWBIO; 1:5,000).Secondary antibody contains horseradish peroxidase-conjugated goat anti-rabbit-IgG (CW0102; CWBIO; 1:5,000) and goat anti-mouse-IgG (CW0103; CWBIO; 1:5,000).
Article Title: ELF5 modulates casein synthesis in goat mammary epithelial cells via JAK2/STAT5 signaling pathway
Article Snippet: Then the membrane incubated with the secondary antibody.Then the membrane incubated with the secondary antibody.. Primary antibodies contains ELF5 (sc-166653, SantaCruz; 1:1,000), αS2-casein (bs-10034R, Bioss; 1:2,000), κ-casein (bs-10031R; Bioss; 1:1,000), αS1-casein (bs-10033R; Bioss; 1:1,000), β-casein (sc-166530; SantaCruz; 1:1,000), p-JAK2 (ab32101; Abcam; 1:1,000), p-STAT5 (9351S; 1:1,000), JAK2 (3230; CST; 1:1,000), STAT5 (610191; BD; 1:1,000), proliferating cell nuclear antigen (PCNA, 10205-2-APl Proteintech, 1:5,000), cyclin dependent kinase 2 (CDK2, 10122-1-AP; Proteintech, 1:10,000), B-cell lymphoma-2-associated X (BAX, 50599-2-lg; Proteintech, 1:10,000), Caspase3 (19677-1-AP; Proteintech, 1:2,000), β-Tubulin (CW0098M; CWBIO, 1:5,000).. Secondary antibody contains horseradish peroxidase-conjugated goat anti-rabbit-IgG (CW0102; CWBIO; 1:5,000) and goat anti-mouse-IgG (CW0103; CWBIO; 1:5,000).Secondary antibody contains horseradish peroxidase-conjugated goat anti-rabbit-IgG (CW0102; CWBIO; 1:5,000) and goat anti-mouse-IgG (CW0103; CWBIO; 1:5,000).
Article Title: Negative regulation of α S1 -casein (CSN1S1) improves β-casein content and reduces allergy potential in goat milk.
Article Snippet: membranes were washed with TBST 3 times, incubated with secondary antibodies. .. Secondary antibody horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (CW0103, CW Biotech; Beijing, China; 1:5,000) was used for primary antibodies αS1-casein (SAB1401093, Sigma-Aldrich; 1:1,000), αS2-casein (bs-10034R, Bioss, Beijing, China; 1:500), β-casein (orb2053, Biorbyt, Cambridgeshire, UK; 1:500), κ-casein (GTX60145, GeneTex, Irvine, CA; 1:500), JAK2 (3230, Cell Signaling Technology, Danvers, MA; 1:1,000), p-JAK2 Tyr1007/1008 (ab32101, Abcam, Cambridge, UK; 1:1,000), mTOR(2972, Cell Signaling Technology; 1:1,000) and p-mTOR Ser2448 (2971; Cell Signaling Technology; 1:1,000). .. Secondary antibody HRP-conjugated goat anti-mouse IgG (CW0102, CW Biotech; 1:5,000)
Negative Control:Article Title: ELF5 modulates casein synthesis in goat mammary epithelial cells via JAK2/STAT5 signaling pathway.
Article Snippet: Then the membrane incubated with the secondary antibody.Then the membrane incubated with the secondary antibody.. Primary antibodies contains ELF5 (sc-166653, SantaCruz; 1:1,000), αS2-casein (bs-10034R, Bioss; 1:2,000), κ-casein (bs-10031R; Bioss; 1:1,000), αS1-casein (bs-10033R; Bioss; 1:1,000), β-casein (sc-166530; SantaCruz; 1:1,000), pJAK2 (ab32101; Abcam; 1:1,000), p-STAT5 (9351S; 1:1,000), JAK2 (3230; CST; 1:1,000), STAT5 (610191; BD; 1:1,000), proliferating cell nuclear antigen (PCNA, 10205-2-APl Proteintech, 1:5,000), cyclin dependent kinase 2 (CDK2, 10122-1-AP; Proteintech, 1:10,000), B-cell lymphoma-2-associated X (BAX, 50599-2-lg; Proteintech, 1:10,000), Caspase3 (19677-1- AP; Proteintech, 1:2,000), β-Tubulin (CW0098M; CWBIO, 1:5,000).. Secondary antibody contains horseradish peroxidase-conjugated goat anti-rabbit-IgG (CW0102; CWBIO; 1:5,000) and goat anti-mouse-IgG (CW0103; CWBIO; 1:5,000).Secondary antibody contains horseradish peroxidase-conjugated goat anti-rabbit-IgG (CW0102; CWBIO; 1:5,000) and goat anti-mouse-IgG (CW0103; CWBIO; 1:5,000).
Article Title: ELF5 modulates casein synthesis in goat mammary epithelial cells via JAK2/STAT5 signaling pathway
Article Snippet: Then the membrane incubated with the secondary antibody.Then the membrane incubated with the secondary antibody.. Primary antibodies contains ELF5 (sc-166653, SantaCruz; 1:1,000), αS2-casein (bs-10034R, Bioss; 1:2,000), κ-casein (bs-10031R; Bioss; 1:1,000), αS1-casein (bs-10033R; Bioss; 1:1,000), β-casein (sc-166530; SantaCruz; 1:1,000), p-JAK2 (ab32101; Abcam; 1:1,000), p-STAT5 (9351S; 1:1,000), JAK2 (3230; CST; 1:1,000), STAT5 (610191; BD; 1:1,000), proliferating cell nuclear antigen (PCNA, 10205-2-APl Proteintech, 1:5,000), cyclin dependent kinase 2 (CDK2, 10122-1-AP; Proteintech, 1:10,000), B-cell lymphoma-2-associated X (BAX, 50599-2-lg; Proteintech, 1:10,000), Caspase3 (19677-1-AP; Proteintech, 1:2,000), β-Tubulin (CW0098M; CWBIO, 1:5,000).. Secondary antibody contains horseradish peroxidase-conjugated goat anti-rabbit-IgG (CW0102; CWBIO; 1:5,000) and goat anti-mouse-IgG (CW0103; CWBIO; 1:5,000).Secondary antibody contains horseradish peroxidase-conjugated goat anti-rabbit-IgG (CW0102; CWBIO; 1:5,000) and goat anti-mouse-IgG (CW0103; CWBIO; 1:5,000).
Article Title: Negative regulation of α S1 -casein (CSN1S1) improves β-casein content and reduces allergy potential in goat milk.
Article Snippet: membranes were washed with TBST 3 times, incubated with secondary antibodies. .. Secondary antibody horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (CW0103, CW Biotech; Beijing, China; 1:5,000) was used for primary antibodies αS1-casein (SAB1401093, Sigma-Aldrich; 1:1,000), αS2-casein (bs-10034R, Bioss, Beijing, China; 1:500), β-casein (orb2053, Biorbyt, Cambridgeshire, UK; 1:500), κ-casein (GTX60145, GeneTex, Irvine, CA; 1:500), JAK2 (3230, Cell Signaling Technology, Danvers, MA; 1:1,000), p-JAK2 Tyr1007/1008 (ab32101, Abcam, Cambridge, UK; 1:1,000), mTOR(2972, Cell Signaling Technology; 1:1,000) and p-mTOR Ser2448 (2971; Cell Signaling Technology; 1:1,000). .. Secondary antibody HRP-conjugated goat anti-mouse IgG (CW0102, CW Biotech; 1:5,000)
Inhibition:Article Title: ELF5 modulates casein synthesis in goat mammary epithelial cells via JAK2/STAT5 signaling pathway.
Article Snippet: Then the membrane incubated with the secondary antibody.Then the membrane incubated with the secondary antibody.. Primary antibodies contains ELF5 (sc-166653, SantaCruz; 1:1,000), αS2-casein (bs-10034R, Bioss; 1:2,000), κ-casein (bs-10031R; Bioss; 1:1,000), αS1-casein (bs-10033R; Bioss; 1:1,000), β-casein (sc-166530; SantaCruz; 1:1,000), pJAK2 (ab32101; Abcam; 1:1,000), p-STAT5 (9351S; 1:1,000), JAK2 (3230; CST; 1:1,000), STAT5 (610191; BD; 1:1,000), proliferating cell nuclear antigen (PCNA, 10205-2-APl Proteintech, 1:5,000), cyclin dependent kinase 2 (CDK2, 10122-1-AP; Proteintech, 1:10,000), B-cell lymphoma-2-associated X (BAX, 50599-2-lg; Proteintech, 1:10,000), Caspase3 (19677-1- AP; Proteintech, 1:2,000), β-Tubulin (CW0098M; CWBIO, 1:5,000).. Secondary antibody contains horseradish peroxidase-conjugated goat anti-rabbit-IgG (CW0102; CWBIO; 1:5,000) and goat anti-mouse-IgG (CW0103; CWBIO; 1:5,000).Secondary antibody contains horseradish peroxidase-conjugated goat anti-rabbit-IgG (CW0102; CWBIO; 1:5,000) and goat anti-mouse-IgG (CW0103; CWBIO; 1:5,000).
Article Title: ELF5 modulates casein synthesis in goat mammary epithelial cells via JAK2/STAT5 signaling pathway
Article Snippet: Then the membrane incubated with the secondary antibody.Then the membrane incubated with the secondary antibody.. Primary antibodies contains ELF5 (sc-166653, SantaCruz; 1:1,000), αS2-casein (bs-10034R, Bioss; 1:2,000), κ-casein (bs-10031R; Bioss; 1:1,000), αS1-casein (bs-10033R; Bioss; 1:1,000), β-casein (sc-166530; SantaCruz; 1:1,000), p-JAK2 (ab32101; Abcam; 1:1,000), p-STAT5 (9351S; 1:1,000), JAK2 (3230; CST; 1:1,000), STAT5 (610191; BD; 1:1,000), proliferating cell nuclear antigen (PCNA, 10205-2-APl Proteintech, 1:5,000), cyclin dependent kinase 2 (CDK2, 10122-1-AP; Proteintech, 1:10,000), B-cell lymphoma-2-associated X (BAX, 50599-2-lg; Proteintech, 1:10,000), Caspase3 (19677-1-AP; Proteintech, 1:2,000), β-Tubulin (CW0098M; CWBIO, 1:5,000).. Secondary antibody contains horseradish peroxidase-conjugated goat anti-rabbit-IgG (CW0102; CWBIO; 1:5,000) and goat anti-mouse-IgG (CW0103; CWBIO; 1:5,000).Secondary antibody contains horseradish peroxidase-conjugated goat anti-rabbit-IgG (CW0102; CWBIO; 1:5,000) and goat anti-mouse-IgG (CW0103; CWBIO; 1:5,000).
Article Title: Negative regulation of α S1 -casein (CSN1S1) improves β-casein content and reduces allergy potential in goat milk.
Article Snippet: membranes were washed with TBST 3 times, incubated with secondary antibodies. .. Secondary antibody horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (CW0103, CW Biotech; Beijing, China; 1:5,000) was used for primary antibodies αS1-casein (SAB1401093, Sigma-Aldrich; 1:1,000), αS2-casein (bs-10034R, Bioss, Beijing, China; 1:500), β-casein (orb2053, Biorbyt, Cambridgeshire, UK; 1:500), κ-casein (GTX60145, GeneTex, Irvine, CA; 1:500), JAK2 (3230, Cell Signaling Technology, Danvers, MA; 1:1,000), p-JAK2 Tyr1007/1008 (ab32101, Abcam, Cambridge, UK; 1:1,000), mTOR(2972, Cell Signaling Technology; 1:1,000) and p-mTOR Ser2448 (2971; Cell Signaling Technology; 1:1,000). .. Secondary antibody HRP-conjugated goat anti-mouse IgG (CW0102, CW Biotech; 1:5,000)
Activity Assay:Article Title: ELF5 modulates casein synthesis in goat mammary epithelial cells via JAK2/STAT5 signaling pathway.
Article Snippet: Then the membrane incubated with the secondary antibody.Then the membrane incubated with the secondary antibody.. Primary antibodies contains ELF5 (sc-166653, SantaCruz; 1:1,000), αS2-casein (bs-10034R, Bioss; 1:2,000), κ-casein (bs-10031R; Bioss; 1:1,000), αS1-casein (bs-10033R; Bioss; 1:1,000), β-casein (sc-166530; SantaCruz; 1:1,000), pJAK2 (ab32101; Abcam; 1:1,000), p-STAT5 (9351S; 1:1,000), JAK2 (3230; CST; 1:1,000), STAT5 (610191; BD; 1:1,000), proliferating cell nuclear antigen (PCNA, 10205-2-APl Proteintech, 1:5,000), cyclin dependent kinase 2 (CDK2, 10122-1-AP; Proteintech, 1:10,000), B-cell lymphoma-2-associated X (BAX, 50599-2-lg; Proteintech, 1:10,000), Caspase3 (19677-1- AP; Proteintech, 1:2,000), β-Tubulin (CW0098M; CWBIO, 1:5,000).. Secondary antibody contains horseradish peroxidase-conjugated goat anti-rabbit-IgG (CW0102; CWBIO; 1:5,000) and goat anti-mouse-IgG (CW0103; CWBIO; 1:5,000).Secondary antibody contains horseradish peroxidase-conjugated goat anti-rabbit-IgG (CW0102; CWBIO; 1:5,000) and goat anti-mouse-IgG (CW0103; CWBIO; 1:5,000).
Article Title: ELF5 modulates casein synthesis in goat mammary epithelial cells via JAK2/STAT5 signaling pathway
Article Snippet: Then the membrane incubated with the secondary antibody.Then the membrane incubated with the secondary antibody.. Primary antibodies contains ELF5 (sc-166653, SantaCruz; 1:1,000), αS2-casein (bs-10034R, Bioss; 1:2,000), κ-casein (bs-10031R; Bioss; 1:1,000), αS1-casein (bs-10033R; Bioss; 1:1,000), β-casein (sc-166530; SantaCruz; 1:1,000), p-JAK2 (ab32101; Abcam; 1:1,000), p-STAT5 (9351S; 1:1,000), JAK2 (3230; CST; 1:1,000), STAT5 (610191; BD; 1:1,000), proliferating cell nuclear antigen (PCNA, 10205-2-APl Proteintech, 1:5,000), cyclin dependent kinase 2 (CDK2, 10122-1-AP; Proteintech, 1:10,000), B-cell lymphoma-2-associated X (BAX, 50599-2-lg; Proteintech, 1:10,000), Caspase3 (19677-1-AP; Proteintech, 1:2,000), β-Tubulin (CW0098M; CWBIO, 1:5,000).. Secondary antibody contains horseradish peroxidase-conjugated goat anti-rabbit-IgG (CW0102; CWBIO; 1:5,000) and goat anti-mouse-IgG (CW0103; CWBIO; 1:5,000).Secondary antibody contains horseradish peroxidase-conjugated goat anti-rabbit-IgG (CW0102; CWBIO; 1:5,000) and goat anti-mouse-IgG (CW0103; CWBIO; 1:5,000).
Article Title: Negative regulation of α S1 -casein (CSN1S1) improves β-casein content and reduces allergy potential in goat milk.
Article Snippet: membranes were washed with TBST 3 times, incubated with secondary antibodies. .. Secondary antibody horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (CW0103, CW Biotech; Beijing, China; 1:5,000) was used for primary antibodies αS1-casein (SAB1401093, Sigma-Aldrich; 1:1,000), αS2-casein (bs-10034R, Bioss, Beijing, China; 1:500), β-casein (orb2053, Biorbyt, Cambridgeshire, UK; 1:500), κ-casein (GTX60145, GeneTex, Irvine, CA; 1:500), JAK2 (3230, Cell Signaling Technology, Danvers, MA; 1:1,000), p-JAK2 Tyr1007/1008 (ab32101, Abcam, Cambridge, UK; 1:1,000), mTOR(2972, Cell Signaling Technology; 1:1,000) and p-mTOR Ser2448 (2971; Cell Signaling Technology; 1:1,000). .. Secondary antibody HRP-conjugated goat anti-mouse IgG (CW0102, CW Biotech; 1:5,000)
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